Company: Case Western Reserve University
Location: Maple Heights
Posted on: September 22, 2023
Working with a high degree of independence under general direction,
the Research Assistant 4 will coordinate major activities related
technical and research areas to work on drugging transcription
through the lens of epigenetics in the 3D genome, using a
combination of biochemical, cell biology, epigenetic and
bioinformatic approaches. These approaches will be applied largely
in the context of cancer. The research assistant will present data
at departmental seminars and national/international scientific
meetings, prepare manuscripts, and contribute preliminary data for
grant proposals. The research assistant will devote time every week
to read the emerging scientific literature surrounding epigenetics,
chromatin regulation, chemical biology, cancer biology, 3D genomics
and nuclear condensate dynamics.
- Provide input and recommendations to principal investigator
regarding significant developments in research projects. Perform
research work in the lab, including molecular biology techniques,
plasmid construction and cloning, PCR/RT-PCR, DNA/RNA sequencing,
western blotting, ChIP-seq, HiChIP, RD-SPRITE, ChRO-seq, library
construction, cell culture, cell line engineering, and lentiviral
- Assist in developing procedures in 3D mapping for the epigenome
and genome. Coordinate information with others as needed. Read,
present and discuss new scientific literature in the field of
chromatin biology and chemical control of transcription. Co-author
research projects and grants, protocol/methods publications, and
primary research articles. (30%)
- Perform the most complex quantitative analytical procedures
using a combination of biochemical, cell biology, epigenetic and
bioinformatic approaches. (10%)
- Coordinate major activities of laboratory; researching
liquid-liquid phase separation properties of nuclear clusters.
Teach or instruct others in research techniques such as but not
limited to PCR/RT-PCR, DNA/RNA sequencing, western blotting,
ChIP-seq. Oversee administrative organization of the laboratory.
May supervise research assistants, technicians, students, and
- Oversee inventory of laboratory supplies related to the
project, ensure order placements one week in advance of running out
of key reagents. Manage laboratory reimbursements and ordering
reconciliation when necessary. Oversee maintenance of equipment
utilized in experiments, such as microscopes for imaging. (4%)
- Perform other duties as assigned. (1%)
Department: Daily contact, meet students in the lab in person to
discuss research projects. Weekly contact with principal
investigator to discuss results and design next steps, coordinate
lab related meetings, budget and lab organization, etc.
University: Occasional contact with other departments on Case
Western Reserve University campus to share information.
External: Occasional contact with vendors to exchange
Students: Weekly contact with student employees regarding research
projects as needs arise.
May supervise incoming and departing researchers (all levels) and
technicians, for appropriate orientation to the culture,
expectations, procedures, and required training models.
Experience: 5 to 8 years of related experience required.
Education: Bachelor's degree in science required.
- Experience with cell culture.
- Experience with biochemical techniques to interrogate proteins
and protein-genome interactions, such as immunoprecipitation
coupled with sequencing (ChIP-seq, HiChIP), RD-SPRITE, and nascent
RNA measurements with ChRO-seq.
- Experience with preparing barcoded libraries of DNA/RNA.
- Proficient with Google sheets and Excel, basic familiarity with
command line and R scripting is a bonus. experiment
- Has knowledge of commonly-used concepts, practices, and
procedures within a particular field.
- Relies on instructions and pre-established guidelines to
perform the functions of the job.
- Ability to operate laboratory equipment.
- Ability to meet consistent attendance.
- Ability to interact with colleagues, supervisors and customers
face to face.
General laboratory environment. May have exposure to formaldehyde,
when fixing cells, and for this a fume hood is provided for safety
protection. Personal protective equipment, including lab coat,
gloves and goggles, will be provided to limit exposture to
In employment, as in education, Case Western Reserve University is
committed to Equal Opportunity and Diversity. Women, veterans,
members of underrepresented minority groups, and individuals with
disabilities are encouraged to apply.
Case Western Reserve University provides reasonable accommodations
to applicants with disabilities. Applicants requiring a reasonable
accommodation for any part of the application and hiring process
should contact the Office of Equity at to request a reasonable
accommodation. Determinations as to granting reasonable
accommodations for any applicant will be made on a case-by-case
Keywords: Case Western Reserve University, Cleveland , Researcher Manager, Executive , Maple Heights, Ohio
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